Related Thesis-中药对照品,中药标准品,化合物库,中药配方颗粒成分检测鉴定,对照药材,化合物定制,成都乐美天科技-乐美天

10-28

2022

Exploration of Habitat-Related Chemical Markers for Stephania tetrandra Applying Multiple Chromatographic and Chemometric Analysis

Abstract Geo-authentic herbs refer to medicinal materials produced in a specific region with superior quality. Stephania tetrandra S. Moore (S. tetrandra) is cultivated in many provinces of China, including Anhui, Zhejiang, Fujian, Jiangxi, Hunan, Guangxi, Guangdong, Hainan, and Taiwan, among which Jiangxi is the geo-authentic origin. To explore habitat-related chemical markers of herbal medicine, an integrated chromatographic technique including gas chromatography-mass spectrometry (GC-MS), ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) combined with chemometric analysis was established. The established methods manifested that they were clearly divided into two groups according to non-authentic origins and geo-authentic origins, suggesting that the metabolites were closely related to their producing areas. A total of 70 volatile compounds and 50 non-volatile compounds were identified in S. tetrandra. Meanwhile, tetrandrine, fangchinoline, isocorydine, magnocurarine, magnoflorine, boldine, and higenamine as chemical markers were accurately quantified and suggested importance in grouping non-authentic origins and geo-authentic origins samples. The discriminatory analysis also indicated well prediction performance with an accuracy of 80%. The results showed that the multiple chromatographic and chemometric analysis technique could be used as an effective approach for discovering the chemical markers of herbal medicine to fulfill the evaluation of overall chemical consistency among samples from different producing areas. ………… 3. Materials and Methods 3.1. Chemical Reagents and Materials Tetrandrine, fangchinoline, magnoflflorine, magnocurarine, isocorydine, higenamine,and boldine were collected from Chengdu DeSiTe Biological Technology Co., Ltd. (Chengdu,China).…… ………… 原文地址：https://doi.org/10.3390/molecules27217224

作者：Xiunan Cao, Xinxin Miao, Minglei Ge……

338

字数：1876

10-28

2022

Drug-polysaccharide/herb interactions and compatibility rationality of Sijunzi decoction based on comprehensive pharmacokinetic screening for multi-components in rats with spleen deficiency syndrome

Abstract Ethnopharmacological relevance Sijunzi decoction (SJZD) is composed of four herbs, namely Ginseng Radix et Rhizoma (RG, Panax ginseng C.A.Mey.), Atractylodes Macrocephalae Rhizoma (AM, Atractylodes macrocephala Koidz.), Poria (Poria cocos (Schw.) Wolf), and Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle (GRP, derived from Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L.) based on the compatibility theory of traditional Chinese medicine (TCM), which is a classical formula for the treatment of spleen deficiency syndrome (SDS) in TCM. The polysaccharides and non-polysaccharides (NPSs) composition represented by flavonoids, saponins and terpenoids are the important pharmacodynamic material basis of SJZD. Aim of the study The aim of this study was to investigate the pharmacokinetic characteristics of SJZD in normal rats and SDS rats, and explore the potential interactions between NPSs and polysaccharides in SJZD, as well as the compatibility rationality of SJZD. ………… 2. Materials and methods 2.1. Materials, reagents, and chemicals ………… Liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, ononin, liquiritigenin, isoliquiritigenin, ginsenoside Rb1 (Rb1), ginsenoside Rc (Rc), formononetin, ginsenoside Rb2 (Rb2),glycyrrhizic acid, ginsenoside Rd (Rd), atractylenolide III, astragaloside, and digoxin, all with purity over 98% (Fig. S1), were purchased from Chengdu DeSiTe Biological Technology Co., Ltd. (Chengdu,China).…… ………… 原文地址：https://doi.org/10.1016/j.jep.2022.115871

作者：Bangjian Dong, Ping Ma……

343

字数：1565

10-19

2022

An Integrated Strategy of Chemical Fingerprint and Network Pharmacology for the Discovery of Efficacy-Related Q-Markers of Pheretima

Abstract Pheretima, one of the animal-derived traditional Chinese medicines, has been wildly used in various cardiovascular and cerebrovascular diseases, including stroke, coronary heart disease, hyperlipidemia, and hyperglycemia. However, it was still a big challenge to select the quality markers for Pheretima quality control. The fingerprint and network pharmacology-based strategy was proposed to screen the efficiency related quality markers (Q-Markers) of Pheretima. The ratio of sample to liquid, ultrasonic-extraction time, temperature, and power were optimized by orthogonal design, respectively. The chemical fingerprint of forty batches of Pheretima was established, and six common peaks were screened. The network pharmacology was used to construct the Pheretima-Components-Targets-Pathways-Stroke network. It was found that six potential efficacy Q-markers in Pheretima could exert the relaxing meridians effect to treat stroke through acting on multiple targets and regulating various pathways. A simple HPLC-DAD method was developed and validated to determine the efficacy Q-markers. Grey relational analysis was used to further verify the relation of potential efficiency related quality markers with the anticoagulation activity of Pheretima, which indicated that the contents of these markers exhibited high relationship with the anticoagulation activity. It was concluded that hypoxanthine, uridine, phenylalanine, inosine, guanosine, and tryptophan were selected as quality markers related to relaxing meridians to evaluate the quality of Pheretima. The fingerprint and network pharmacology-based strategy was proved to be a powerful strategy for the discovery of efficiency related Q-markers of Pheretima. ………… 2. Materials and Methods 2.1. Chemicals and Reagent Hypoxanthine, uridine, adenine, inosine, guanosine, and adenosine were purchased from Chengdu Desite Bio-Technology Co., Ltd. (Chengdu, China). ………… 原文地址：https://doi.org/10.1155/2022/8774913

作者：Ye Shang，Suyi Liu……

361

字数：1890

10-19

2022

Quantitative Comparison and Chemical Profile Analysis of Different Medicinal Parts of Perilla frutescens (L.) Britt. from Different Varieties and Harvest Periods

　　ABSTRACT: Perilla frutescens (L.) Britt. is a plant that has been classified as one of the “One Root of Medicine and Food”, and it can be used both as medicine and as food. To explore the influence of different varieties and harvest periods on the quality of different medicinal parts of P. frutescens, a comprehensive study on the chemical constituents of P. frutescens based on plant metabolomics was conducted. A total of 57 nonvolatile chemical components and 105 volatile chemical components of P. frutescens were characterized by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) and gas chromatography-mass spectrometry (GC-MS). Furthermore, 35, 27, and 2 nonvolatile constituents as well as 16, 16, and 18 volatile constituents were identified as potential markers for discriminating P. frutescens between different medicinal parts, different varieties, and different harvest periods, respectively. Besides, 22 bioactive compounds of P. frutescens were quantitatively determined by a new sensitive UPLC-MS/MS method. This study comprehensively compares the differences and similarities of P. frutescens among the different medicinal parts, different varieties, and different harvest periods, and the results of this study may provide a theoretical basis and guidance for studying the quality evaluation and the optimization of the harvest period of this plant. ………… 2. MATERIAL AND METHODS Adenosine, salvianic, protocatechuic acid, protocatechnic aldehyde,caffeic acid, scopoletin, luteolin-7-O-glucuronide, scutellarin, trypto-phan, chlorogenic acid, apigenin-7-glucoside, methyl rosmarinate,apigenin-7-O-glucuronide, quercetin, luteolin, apigenin, tormentic acid, betulonic acid, cynaroside, rutin, ferulic acid, and rosmarinic acid (purity of all standards ≥ 98%) were purchased from Chengdu Desite Biotechnology Co., Ltd. (Chengdu, China). …… ………… Complete contact information is available at: https://pubs.acs.org/10.1021/acs.jafc.2c03104

作者：Yuqi Fan, Xiunan Cao, Mengmeng Zhang,……

384

字数：1870

09-28

2022

Resveratrol reverses TGF-β1-mediated invasion and metastasis of breast cancer cells via the SIRT3/AMPK/autophagy signal axis

Abstract Resveratrol (Resv) has antitumorigenic and antimetastatic activities; however, the molecular mechanisms underlying the inhibitory effects of Resv on the invasion and metastasis of breast cancer cells are still a subject of debate. In our study, we demonstrated that Resv inhibited tumor cell proliferation and tumor growth. It also suppressed invasion and pulmonary metastasis of breast cancer by reversing the transforming growth factor beta 1 (TGF-β1)-mediated EMT process. Meanwhile, the anticarcinogenic effects of Resv were abolished by the autophagy blocker 3-methyladenine (3-MA) or Beclin 1 small interfering RNA. Moreover, Resv upregulated autophagy-related genes and protein levels and induced the formation of autophagosomes in 4T1 breast cancer cells and xenograft mice, suggesting that autophagy was involved in the anticarcinogenic activities of Resv in bothmodels. In addition, Resv-induced autophagy by increasing the expression of SIRT3 and phosphorylated AMPK. SIRT3 knockdown reduced AMPK phosphorylation and autophagy-related proteins levels, and suppressed the anticancer effects of Resv, demonstrating that the inhibitory effects of Resv on tumor progression were medi ated via the SIRT3/AMPK/autophagy pathway. Taken together, our study provided novel insight into the anticancer effects of Resv and revealed that targeting the SIRT3/AMPK/autophagy pathway can serve as a new therapeutic target against breast cancer. ...... Chemicals and reagents Recombinant human TGF-β1 protein was bought from Peprotech (Rocky Hill, NJ, USA). Resveratrol (CAS number 501-36-0, purity ≥98.0%), identified as pure trans-resveratrol was supplied by Chengdu DeSiTe Biological Technology Co., Ltd (Chengdu, China). DOI: 10.1002/ptr.7608

作者：乐美天小编

422

字数：1605

09-19

2022

World Journal of Microbiology and Biotechnology-20 June 2018

期刊名：World Journal of Microbiology and Biotechnology文献编号：DOI 10.1007/s11274-018-2464-1文献地址：https://link.springer.com/article/10.1007%2Fs13318-017-0444-8发表日期：20 June 2018 Abstract The present study has focused on the effects of hypericin (Hyp) based photodynamic inactivation (PDI) of Escherichia coli (E. coli). To evaluate the efficiency of Hyp based PDI of E. coli, single factor experiments and response surface optimization experiment were conducted to obtain the optimum parameter values (36 µM Hyp, 5.9 J cm−2 light dose: 16.4 mW cm−2, 60 W, 260 s, 590 nm and 68 min incubation time) and finally achieved a 4.1 log CFU mL−1 decrease of E. coli. Cell-Hyp interaction and intracellular reactive oxygen species (ROS) level were detected by fluorescence spectrometric photometer. Data indicated that Hyp possessed a strong ability to bind with cells. In addition, a significant increase was observed in intracellular ROS level after Hyp-based photosensitization treatment. Therefore, Hyp-based photosensitization seems to be a promising method to efficiently inactivate E. coli. It is expected to be a safe, efficient, low cost and practical method which can be applied in the field of food safety. Hypericin used as a photosensitizer in present study was obtained from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China).

作者：未知

917

字数：1297

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World Journal of Microbiology and Biotechnology-20 June 2018