Chlorogenic acid ameliorates mice clinical endometritis ...

Release time：2022-09-16

Author：乐美天标准品

Reading times：519

Words：1239

期刊名：Journal of Pharmacy and Pharmacology
期刊网址：https://pubmed.ncbi.nlm.nih.gov/33734387/
发表日期：18 March 2021bstract

Objectives Clinical endometritis is a common reproductive disorder in mammals that seriously

endangers animal health and causes economic losses worldwide. This study aims to use lipopoly

saccharide and Trueperella pyogenes exotoxin as modelling reagents (LC) to perfuse the mouse

uterus in order to establish a model of clinical endometritis and to investigate the anti-inflamma

tory and antioxidant effects of chlorogenic acid (CGA).

Methods In this study, five LC uterine perfusions were selected to model clinical endomet

ritis. The anti-inflammatory and antioxidant effects of CGA were clarified. Through HE staining,

proinflammatory cytokines, blood testing, NFκB and Keap1/Nrf2 signalling pathways and other

index changes to explore the protection mechanism of CGA.

Key findings After CGA treatment, the appearance, inflammatory damage and blood indicators of

the mouse uterus returned to normal. Simultaneously, CGA could inhibit the activation of NFκB

and reduce the release of inflammatory cytokines; CGA could also activate Keap1/Nrf2, promote

the dissociation of Keap1 and Nrf2 and significantly increase the expression of the downstream

genes HO-1 and NQO1.

CGA (purity >98.0%) was purchased from Desite Bio (Chengdu, China).

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