Journal of Pharmaceutical and Biomedical Analysis

Comparison of the active components of Aster tataricus from different regions and related processed products by ultra-high performance liquid chromatography with tandem mass spectrometry
期刊名：Journal of separation science
文献编号：DOI 10.1002/jssc.201900814
文献地址：https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201900814
发表日期：07 January 2020

Abstract
We investigated crude Aster tataricus, vinegar‐processed Aster tataricus, honey‐processed Aster tataricus, and steamed Aster tataricus as a case study and developed a comprehensive strategy integrating quantitative analysis and chemical pattern recognition methods for the evaluation and differentiation of Aster tataricus from different regions, as well as related processed products. In the study, 15 batches of raw Aster tataricus collected from seven provinces were analyzed. A sensitive and rapid ultra‐high performance liquid chromatography with tandem mass spectrometry method for simultaneous determination of 15 compounds was established to evaluate the quality of raw and processed Aster tataricus. Furthermore, multivariate statistical techniques were applied to compare the differences among Aster tataricus samples. As a result, the herbs collected from seven provinces were divided into two categories, and chlorogenic acid was the most important component distinguishing between the regions. Moreover, all of the raw and processed samples were classified by partial least squares discriminant analysis based on the 15 analyzed compounds. Results showed that raw Aster tataricus, vinegar‐processed Aster tataricus, honey‐processed Aster tataricus, and steamed Aster tataricus were clustered in four different areas. Shionone, chlorogenic acid and kaempferol were the significant constituents differentiating the raw and differently processed Aster tataricus samples.

Luteolin, shionone, quercetin, scopoletin, kaempferol, kaempferol-7-O-β-D-glucopyranoside, isorhamnetin, 7-hydroxycoumarin, methyl caffeate, and protocatechuic acid (purity ≥ 98%) were purchased from Chengdu Desite Biological Technology Co. Ltd. (Sichuan, China)